Unraveling the Role of Heme Oxygenase-1 (HO-1) in Prostate Cancer progression; an RNA Sequencing Approach
Presenter Type
UNO Undergraduate Student
Major/Field of Study
Bioinformatics
Advisor Information
Associate Professor/ Undergraduate Program Chair, BIOI, CYBR, and ITIN
Location
CEC RM #201/205/209
Presentation Type
Poster
Poster Size
36"x24"
Start Date
22-3-2024 2:30 PM
End Date
22-3-2024 3:45 PM
Abstract
Prostate cancer (PC) is one of the leading causes of cancer related death among men on the global scale. In the US, almost 300,000 new cases are expected every year, and PC remains the second most common cancer in men worldwide, accounting for 15.1% of all cancers diagnosed in 2020. Heme oxygenase-1 (HO-1) is an enzyme known for its antioxidant and anti-inflammatory properties, making it an important enzyme in cellular stress responses. The cytoprotective effects of HO-1 are mediated through scavenging Reactive Oxygen Species, and regulating inflammation, as well as apoptosis. The Abdalla lab at the University of Nebraska Medical Center is dedicated to uncovering the role of HO-1 in PC response to chemotherapy, and its role in PC progression and metastasis. They conducted bulk RNA sequencing on HO-1 knockout PC cell lines to compare them against the parental cells. This comparison will provide insights into the downstream effects of HO-1 on various aspects of prostate cancer, including disease development, metastasis, and therapeutic response. In this study, I have received the bulk RNA sequence dataset from the Abdalla lab to perform the cell line comparison. As part of the pipeline, I have performed quality assessment for the raw RNA sequence, sequence processing to remove adapters, as well as sequence alignment. The outcome of this computational pipeline will be a summary of genetic variants and annotations on their impact in HO-1 knockouts versus parental controls.
Unraveling the Role of Heme Oxygenase-1 (HO-1) in Prostate Cancer progression; an RNA Sequencing Approach
CEC RM #201/205/209
Prostate cancer (PC) is one of the leading causes of cancer related death among men on the global scale. In the US, almost 300,000 new cases are expected every year, and PC remains the second most common cancer in men worldwide, accounting for 15.1% of all cancers diagnosed in 2020. Heme oxygenase-1 (HO-1) is an enzyme known for its antioxidant and anti-inflammatory properties, making it an important enzyme in cellular stress responses. The cytoprotective effects of HO-1 are mediated through scavenging Reactive Oxygen Species, and regulating inflammation, as well as apoptosis. The Abdalla lab at the University of Nebraska Medical Center is dedicated to uncovering the role of HO-1 in PC response to chemotherapy, and its role in PC progression and metastasis. They conducted bulk RNA sequencing on HO-1 knockout PC cell lines to compare them against the parental cells. This comparison will provide insights into the downstream effects of HO-1 on various aspects of prostate cancer, including disease development, metastasis, and therapeutic response. In this study, I have received the bulk RNA sequence dataset from the Abdalla lab to perform the cell line comparison. As part of the pipeline, I have performed quality assessment for the raw RNA sequence, sequence processing to remove adapters, as well as sequence alignment. The outcome of this computational pipeline will be a summary of genetic variants and annotations on their impact in HO-1 knockouts versus parental controls.