Author

Gang Wu

Date of Award

6-1-1994

Document Type

Thesis

Degree Name

Master of Arts (MA)

Department

Biology

First Advisor

Dr. Bruce Chase

Abstract

In the central nervous system (CNS) of insects and other invertebrates, acetylcholine (Ach) functions as a major neurotransmitter and acts upon a number of different types of receptors. One approach is to the identification of acetylcholine receptors (AChR) is to use cross-homology between vertebrate and invertebrate AChRs. To this end, monoclonal antibodies (mAbs) to the well-characterized nicotinic vertebrate AChR that cross-react with Drosophila tissues have been developed. One of them, mAb 16.42, was used to screen a lambda-ZAP expression vector library in which Drosophila adult head cDNAs were inserted, and three cDNAs that may encode nAChRs were identified.

Comments

A Thesis Presented to the Department of Biology and the Faculty of the Graduate College University of Nebraska In Partial Fulfillment of the Requirements for the Degree Master of Arts University of Nebraska at Omaha. Copyright 1994 Gang Wu.

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