Document Type

Article

Publication Date

8-28-2012

Publication Title

Journal of Chemical Education

Volume

89

Issue

11

First Page

1458

Last Page

1460

Abstract

This laboratory experiment, using proton NMR spectroscopy to determine the dissociation constant for heterocyclic bases, has been modified from a previously described experiment. A solution of a substituted pyridine is prepared using deuterium oxide (D2O) as the solvent. The pH of the solution is adjusted and proton NMR spectra are collected for a variety of pH values. The chemical shifts of the peaks in the NMR spectrum change depending on the degree of protonation of the pyridine ring. Analysis of the spectral data is used to calculate the dissociation constant of the substituted pyridine. This experiment is suitable for a variety of advanced chemistry laboratories including analytical chemistry and instrumental analysis.

Comments

Copyright © 2012 The American Chemical Society and Division of Chemical Education, Inc.

Uploaded to this site is the post-print version of the article. The published version of the article can be found here.
DOI: 10.1021/ed200433z

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