Document Type

Article

Publication Date

2-26-2001

Publication Title

Journal of Biochemical and Biophysical Methods

Volume

47

Issue

3

First Page

189

Last Page

195

Abstract

Fluorescent labeling of primary and secondary amines using dansyl chloride has been widely used in the past. Its application provides an extremely sensitive means to detect amine functional groups to amounts of less than 1 mg of material. This work describes a method for the dansylation of hydroxyl (–OH) and carboxylic acid (–COOH) functional groups. This technique is demonstrated with ethanol, gamma hydroxy butyric acid (GHB), benzoic acid, and p-chloroaniline. Sensitivity of detection for all compounds are microgram or microliter. For the compounds ethanol and GHB which are liquids at room temperature, as little as 1 ml quantity can be detected. Benzoic acid and p-chloroaniline which are solids at room temperature can be detected at levels of 1 mg. Fast thin layer chromatography was accomplished using acetone as the resolving solvent, which resulted in good differentiation of analytes for Rr measurement. The dansylation reaction performed similarly at pH 11, 10 and 9.6 and uses 2 molar Na2 CO3.

Comments

This is an Accepted Manuscript of an article published by Elsevier in Journal of Biochemical and Biophysical Methods on February 26, 2001, available online: https://doi.org/10.1016/S0731-7085(02)00168-1

Creative Commons License

Creative Commons Attribution-No Derivative Works 4.0 License
This work is licensed under a Creative Commons Attribution-No Derivative Works 4.0 License.

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