Advisor Information
Jodi Kreiling
Location
Dr. C.C. and Mabel L. Criss Library
Presentation Type
Poster
Start Date
3-3-2017 12:30 PM
End Date
3-3-2017 1:45 PM
Abstract
The mannose-6-phosphate/insulin-like growth factor 2 receptor (M6P/IGF2R), a recombinant triplet protein receptor for studies involving receptor dimerization, plays a pivotal role in mediating cellular growth. This receptor is comprised of fifteen similar units that fold into five triplet subunits. The goal of this experiment is to investigate how to make the 1-3MH protein more stable at the DNA level and generate a larger yield from DNA extractions for more effective transfections. This was accomplished by minorly changing the protocols for the DNA purification. Instead of using a standard column DNA purification kit, a crude DNA purification was done instead. This increased the DNA yield from less than 1 μg/μL to approximately 4-5 μg/μL. Western blot analysis ultimately showed that the increase in DNA concentration prior to transfection did not improve the expression of protein.
Characterization of the Mannose-6-Phosphate/Insulin-like Growth Factor II Receptor Using 1-3MH
Dr. C.C. and Mabel L. Criss Library
The mannose-6-phosphate/insulin-like growth factor 2 receptor (M6P/IGF2R), a recombinant triplet protein receptor for studies involving receptor dimerization, plays a pivotal role in mediating cellular growth. This receptor is comprised of fifteen similar units that fold into five triplet subunits. The goal of this experiment is to investigate how to make the 1-3MH protein more stable at the DNA level and generate a larger yield from DNA extractions for more effective transfections. This was accomplished by minorly changing the protocols for the DNA purification. Instead of using a standard column DNA purification kit, a crude DNA purification was done instead. This increased the DNA yield from less than 1 μg/μL to approximately 4-5 μg/μL. Western blot analysis ultimately showed that the increase in DNA concentration prior to transfection did not improve the expression of protein.