The analysis of stimulated white blood cells from common marmosets (Callithrix jacchus) using a human biomarker panel
Presenter Type
UNO Undergraduate Student
Major/Field of Study
Biology
Other
Biology
Advisor Information
Jonathan Clayton
Location
MBSC304 - U
Presentation Type
Oral Presentation
Start Date
24-3-2023 1:00 PM
End Date
24-3-2023 2:15 PM
Abstract
The analysis of stimulated white blood cells from common marmosets (Callithrix jacchus) using a human biomarker panel
Aliyah Jabenis1,2, Donald DJ Rogers1, Mackenzie Conrin1,2, Shivdeep S. Hayer1,2, Haley R. Hassenstab2, Noel D. Johnson3, Paul W. Denton1, and Jonathan B. Clayton1,2,4
1Department of Biology, University of Nebraska at Omaha
2Callitrichid Research Center, University of Nebraska at Omaha
3Department of Comparative Medicine, University of Nebraska Medical Center
4Nebraska Food for Health Center, University of Nebraska-Lincoln
Common marmosets (Callithrix jacchus) have been a useful translational model for humans due to similarities in social behavior and their prefrontal cortex. Although marmosets are a common and beneficial animal model for biomedical purposes, they lack species-specific tests designed to acquire data on their biological functions. Because of this, it is necessary to validate and use existing human-specific assays. A primary focus of our research group is the gut-brain-immune axis, and understanding the link between these systems. However, in order to fully study this system we must first validate the use of assays designed for humans using marmoset biological (i.e., blood, urine, and feces) samples. The primary focus of this is the generation and detection of inflammatory analytes in marmosets using a commercially available human immunoassay. For future reference material, we tested stimulated (by lipopolysaccharide or PMA/Ionomycin) mononuclear white blood cells from marmosets against a Mesoscale Diagnostics (MSD) 4 plate V-PLEX Custom Human Biomarker panel. This included a pro-inflammatory panel, cytokine panel, chemokine panel, angiogenesis panel, and vascular injury panel. Some analytes, such as VEGF and IL-12, definitively cross-reacted with marmoset cell culture supernatants. Other analytes (e.g., IFN-g) either need further testing or are not detected at all (e.g., IL-10). Going forward, we plan to run this validation on a larger scale to develop a method by which we can use a human-specific MSD assays to detect cytokines from a marmoset cell stimulation protocol. Results generated from these experiments can be used in conjunction with behavioral observations and microbiome data to expand our knowledge of the connection between the digestive, nervous, and immune systems.
Scheduling
1-2:15 p.m., 2:30 -3:45 p.m.
The analysis of stimulated white blood cells from common marmosets (Callithrix jacchus) using a human biomarker panel
MBSC304 - U
The analysis of stimulated white blood cells from common marmosets (Callithrix jacchus) using a human biomarker panel
Aliyah Jabenis1,2, Donald DJ Rogers1, Mackenzie Conrin1,2, Shivdeep S. Hayer1,2, Haley R. Hassenstab2, Noel D. Johnson3, Paul W. Denton1, and Jonathan B. Clayton1,2,4
1Department of Biology, University of Nebraska at Omaha
2Callitrichid Research Center, University of Nebraska at Omaha
3Department of Comparative Medicine, University of Nebraska Medical Center
4Nebraska Food for Health Center, University of Nebraska-Lincoln
Common marmosets (Callithrix jacchus) have been a useful translational model for humans due to similarities in social behavior and their prefrontal cortex. Although marmosets are a common and beneficial animal model for biomedical purposes, they lack species-specific tests designed to acquire data on their biological functions. Because of this, it is necessary to validate and use existing human-specific assays. A primary focus of our research group is the gut-brain-immune axis, and understanding the link between these systems. However, in order to fully study this system we must first validate the use of assays designed for humans using marmoset biological (i.e., blood, urine, and feces) samples. The primary focus of this is the generation and detection of inflammatory analytes in marmosets using a commercially available human immunoassay. For future reference material, we tested stimulated (by lipopolysaccharide or PMA/Ionomycin) mononuclear white blood cells from marmosets against a Mesoscale Diagnostics (MSD) 4 plate V-PLEX Custom Human Biomarker panel. This included a pro-inflammatory panel, cytokine panel, chemokine panel, angiogenesis panel, and vascular injury panel. Some analytes, such as VEGF and IL-12, definitively cross-reacted with marmoset cell culture supernatants. Other analytes (e.g., IFN-g) either need further testing or are not detected at all (e.g., IL-10). Going forward, we plan to run this validation on a larger scale to develop a method by which we can use a human-specific MSD assays to detect cytokines from a marmoset cell stimulation protocol. Results generated from these experiments can be used in conjunction with behavioral observations and microbiome data to expand our knowledge of the connection between the digestive, nervous, and immune systems.