Phenotypic characterization of wildtype and mutant Glechoma hederacea.

Presenter Information

Makenzie MeeganFollow

Presenter Type

UNO Undergraduate Student

Major/Field of Study

Biology

Author ORCID Identifier

0000-0002-8888-3658

Advisor Information

Research Mentor

Location

MBSC Ballroom Poster # 705 - U

Presentation Type

Poster

Start Date

24-3-2023 10:30 AM

End Date

24-3-2023 11:45 AM

Abstract

Phenotypes are visual or measurable features of an organism. At times a single gene or mutation will control the expression of many different phenotypes: this is known as pleiotropy. An example of pleiotropy in humans is Marfan syndrome, a genetic condition that affects the heart, eyes, blood vessels, and skeleton. Genetic variation is what allows for differences among individuals within a species. Variation is also responsible for determining what phenotype is expressed. Phenotypic variation in plants can result from either genetic or environmental differences.

Thus far, comparisons have been made between the VirL genotype and a single locally acquired wildtype Glechoma hederacea. To confirm that differences between these can be attributed to the mutation, it is necessary to determine the range of phenotypes occurring in non-mutant plants. Phenotypes that appear to differ between the VirLmutant and wildtype G. hederacea include rate of chloroplast maturation and accompanying pigmentation, leaf soluble protein content, leaf dry matter accumulation, leaf malate dehydrogenase activity, leaf venation patterns and lobe numbers, and nodal trichome numbers and structures. In this study we will attempt to determine whether these “mutant phenotypes” lie outside the naturally occurring range of phenotypes among wildtype accessions from around the Omaha metropolitan area. We have since collected the accessions from different sites around the metropolitan area. In the future, we plan to review the comparisons made on a larger population of the wildtype G. hederacea. We expect to see the same results when comparing the Virl mutant with a larger range of wildtype G. hederacea. From that, we would propose that phenotypes associated with the mutant that fall outside the range of wildtype phenotypic variability might be direct or indirect consequences of the VirL mutation: a condition called pleiotropy.

Scheduling

10:45 a.m.-Noon, 1-2:15 p.m., 2:30 -3:45 p.m.

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Mar 24th, 10:30 AM Mar 24th, 11:45 AM

Phenotypic characterization of wildtype and mutant Glechoma hederacea.

MBSC Ballroom Poster # 705 - U

Phenotypes are visual or measurable features of an organism. At times a single gene or mutation will control the expression of many different phenotypes: this is known as pleiotropy. An example of pleiotropy in humans is Marfan syndrome, a genetic condition that affects the heart, eyes, blood vessels, and skeleton. Genetic variation is what allows for differences among individuals within a species. Variation is also responsible for determining what phenotype is expressed. Phenotypic variation in plants can result from either genetic or environmental differences.

Thus far, comparisons have been made between the VirL genotype and a single locally acquired wildtype Glechoma hederacea. To confirm that differences between these can be attributed to the mutation, it is necessary to determine the range of phenotypes occurring in non-mutant plants. Phenotypes that appear to differ between the VirLmutant and wildtype G. hederacea include rate of chloroplast maturation and accompanying pigmentation, leaf soluble protein content, leaf dry matter accumulation, leaf malate dehydrogenase activity, leaf venation patterns and lobe numbers, and nodal trichome numbers and structures. In this study we will attempt to determine whether these “mutant phenotypes” lie outside the naturally occurring range of phenotypes among wildtype accessions from around the Omaha metropolitan area. We have since collected the accessions from different sites around the metropolitan area. In the future, we plan to review the comparisons made on a larger population of the wildtype G. hederacea. We expect to see the same results when comparing the Virl mutant with a larger range of wildtype G. hederacea. From that, we would propose that phenotypes associated with the mutant that fall outside the range of wildtype phenotypic variability might be direct or indirect consequences of the VirL mutation: a condition called pleiotropy.