Using common marmoset (Callithrix jacchus) feces to isolate and characterize Escherichia coli
Presenter Type
UNO Undergraduate Student
Major/Field of Study
Biology
Other
Biology
Advisor Information
Jonathan B. Clayton
Location
MBSC Ballroom Poster # 805 - U
Presentation Type
Poster
Start Date
24-3-2023 10:30 AM
End Date
24-3-2023 11:45 AM
Abstract
Using common marmoset (Callithrix jacchus) feces to isolate and characterize Escherichia coli
Andrew K. Huang1, Emma Sorrell1, Paul Ayayee1, Aliyah Jabenis1,2, and Jonathan B. Clayton1,2,3,4
1Department of Biology, University of Nebraska at Omaha, Omaha, NE, USA
2Callitrichid Research Center, University of Nebraska at Omaha, Omaha, NE, USA
3Nebraska Food for Health Center, University of Nebraska-Lincoln, Lincoln, NE, USA
4Primate Microbiome Project, University of Nebraska-Lincoln, Lincoln, NE, USA
The mammalian gut microbiome is home to a conglomerate of diverse bacteria which collectively function as an organ capable of influencing the host organism’s metabolic outcomes. One of the most well-known members of the mammalian gut microbiome is Escherichia coli (E. coli), a gram-negative bacterium that typically resides in the lower intestine. Fecal samples collected from common marmosets housed at the Callitrichid Research Center (CRC) located at the University of Nebraska at Omaha were used to develop an effective and reproducible E. coli culture protocol. Factors such as choice of nutrient broth, selective agar, rpm speed of the shaking incubator, and glycerol concentration were manipulated for this purpose. We recorded details regarding growth, viability, and presence/absence of contamination for all cultures. Our results indicate that a protocol involving an initial growth period of 18-24 hours in buffered peptone water facilitated by a shaking incubator set at 37° C and 125 rpm, followed by rounds of four-quadrant streaking on MacConkey (MAC) agar until pure colonies are achieved, followed by four-quadrant streaking on Eosin Methylene Blue (EMB) agar, and storage of culture media in a 10-15% glycerol concentration, consistently yields viable E. coli cultures. Expansion of the E. coli strain library is currently ongoing. Once completed, we plan to utilize the strain library for a series of endeavors, including a study focused on examining antibiotic resistance profiles of the marmoset-derived E. coli.
Scheduling
10:45 a.m.-Noon, 1-2:15 p.m., 2:30 -3:45 p.m.
Using common marmoset (Callithrix jacchus) feces to isolate and characterize Escherichia coli
MBSC Ballroom Poster # 805 - U
Using common marmoset (Callithrix jacchus) feces to isolate and characterize Escherichia coli
Andrew K. Huang1, Emma Sorrell1, Paul Ayayee1, Aliyah Jabenis1,2, and Jonathan B. Clayton1,2,3,4
1Department of Biology, University of Nebraska at Omaha, Omaha, NE, USA
2Callitrichid Research Center, University of Nebraska at Omaha, Omaha, NE, USA
3Nebraska Food for Health Center, University of Nebraska-Lincoln, Lincoln, NE, USA
4Primate Microbiome Project, University of Nebraska-Lincoln, Lincoln, NE, USA
The mammalian gut microbiome is home to a conglomerate of diverse bacteria which collectively function as an organ capable of influencing the host organism’s metabolic outcomes. One of the most well-known members of the mammalian gut microbiome is Escherichia coli (E. coli), a gram-negative bacterium that typically resides in the lower intestine. Fecal samples collected from common marmosets housed at the Callitrichid Research Center (CRC) located at the University of Nebraska at Omaha were used to develop an effective and reproducible E. coli culture protocol. Factors such as choice of nutrient broth, selective agar, rpm speed of the shaking incubator, and glycerol concentration were manipulated for this purpose. We recorded details regarding growth, viability, and presence/absence of contamination for all cultures. Our results indicate that a protocol involving an initial growth period of 18-24 hours in buffered peptone water facilitated by a shaking incubator set at 37° C and 125 rpm, followed by rounds of four-quadrant streaking on MacConkey (MAC) agar until pure colonies are achieved, followed by four-quadrant streaking on Eosin Methylene Blue (EMB) agar, and storage of culture media in a 10-15% glycerol concentration, consistently yields viable E. coli cultures. Expansion of the E. coli strain library is currently ongoing. Once completed, we plan to utilize the strain library for a series of endeavors, including a study focused on examining antibiotic resistance profiles of the marmoset-derived E. coli.