Employing an experimental approach to study the killing capacity of natural killer cells
Presenter Type
UNO Undergraduate Student
Other
Molecular and Biomedical Biology
Advisor Information
Dr. Paul W Denton
Location
CEC RM #231
Presentation Type
Oral Presentation
Start Date
22-3-2024 10:30 AM
End Date
22-3-2024 11:45 AM
Abstract
Employing an experimental approach to study the killing capacity of human natural killer cells
Diya Joy Varughese1, Paul W Denton, PhD1
1Department of Biology, University of Nebraska at Omaha
The Denton Immunobiology Laboratory recently published a novel experimental approach in Heliyon. In this approach, the authorship group provided a strategy for assessing the killing ability of human natural killer (NK) cells ex vivo. The authors gave the assay the name NK SADKA which is an acronym for Natural Killer cell Simultaneous ADCC and Direct Killing Assay. The simultaneous in the name refers to the fact that this approach allows for a single human donor be tested in two different contexts of killing diseased “target” cells. The first context is direct killing – where NK cells recognize target cells based on a “missing signal”. The second context is ADCC (antibody-dependent cell-mediated cytotoxicity) – where NK cells recognize target cells with help from an antibody. In this talk, the basic assay will be presented. Then, the focus will be on the range of possibilities that are available for implementing this assay in the context of pre-clinical drug evaluations. Example applications for employing the NK-SADKA include the evaluation of immunotherapy intervention impacts on NK cell killing function. With the NK-SADKA, it is possible to know whether such intervention impact both killing functions by NK cells equally or not.
Employing an experimental approach to study the killing capacity of natural killer cells
CEC RM #231
Employing an experimental approach to study the killing capacity of human natural killer cells
Diya Joy Varughese1, Paul W Denton, PhD1
1Department of Biology, University of Nebraska at Omaha
The Denton Immunobiology Laboratory recently published a novel experimental approach in Heliyon. In this approach, the authorship group provided a strategy for assessing the killing ability of human natural killer (NK) cells ex vivo. The authors gave the assay the name NK SADKA which is an acronym for Natural Killer cell Simultaneous ADCC and Direct Killing Assay. The simultaneous in the name refers to the fact that this approach allows for a single human donor be tested in two different contexts of killing diseased “target” cells. The first context is direct killing – where NK cells recognize target cells based on a “missing signal”. The second context is ADCC (antibody-dependent cell-mediated cytotoxicity) – where NK cells recognize target cells with help from an antibody. In this talk, the basic assay will be presented. Then, the focus will be on the range of possibilities that are available for implementing this assay in the context of pre-clinical drug evaluations. Example applications for employing the NK-SADKA include the evaluation of immunotherapy intervention impacts on NK cell killing function. With the NK-SADKA, it is possible to know whether such intervention impact both killing functions by NK cells equally or not.