Date of Award
5-1-1996
Document Type
Thesis
Degree Name
Master of Arts (MA)
Department
Biology
First Advisor
Dr. A. Thomas Weber
Abstract
Previously a transcript corresponding to a gene in Dictyostelium mucoroides designated A-11 was found in amoebae during vegetative growth and macrocyst formation. The derived amino acid sequence of A-11 was used to search Genbank to find homologous proteins. The highest degree of homology was found with a hemoglobin from the bacterium Vitreoscilla. The Vitreoscilla hemoglobin protein showed greater than 65% similarity and 50% identity with the A-11 amino acid sequence. It was reported that hypoxic conditions could stimulate the expression of the gene corresponding to the Vitreoscilla hemoglobin when cloned in E. coli cells.
Recommended Citation
Kosinski, Mary E., "The effects of hypoxic conditions on Dictyostelium mucoroides." (1996). Student Work. 3308.
https://digitalcommons.unomaha.edu/studentwork/3308
Comments
A Thesis Presented to the Department of Biology and the Faculty of the Graduate College University of Nebraska In Partial Fulfillment of the Requirements for the Degree Master of Arts University of Nebraska at Omaha. Copyright 1996 Mary E. Kosinski.