A methodology for co-registering the intravascular ultrasound (IVUS) images with the histology sections in the peripheral vasculature
Presenter Type
UNO Graduate Student (Masters)
Major/Field of Study
Biomechanics
Other
Biomechanics
Advisor Information
Assistant Professor
Location
MBSC Ballroom Poster # 1202 - G (Masters)
Presentation Type
Poster
Start Date
24-3-2023 9:00 AM
End Date
31-3-2023 10:15 AM
Abstract
Peripheral vascular disease (PAD), observed primarily in the femoropopliteal artery (FPA), affects over 200 million people worldwide. This vascular disease reduces blood flow, claudication, and gangrene, which remains a substantial public health burden. Intravascular Ultrasound (IVUS) is an imaging technique that uses ultrasound waves to accurately visualize the vascular structure, providing information about the arterial plaque and its composition, which helps the clinician with diagnosis and choosing the appropriate treatment approach. Virtual Histology IVUS (VH-IVUS) provides a more detailed assessment of plaque composition through spectral analysis of high-frequency ultrasound signals compared to greyscale IVUS, which remains less accurate in differentiating the heterogeneous plaque compositions. The VH-IVUS has been studied extensively in coronary and carotid arteries. However, there has yet to be a large-scale validation study that cross-references IVUS observations with conventional histology images in FPA. In this preliminary study, we aimed to develop a setup to image FPAs ex vivo using IVUS, micro Computed Tomography (microCT), and conventional histology in a loading condition similar to in vivo. To achieve this goal, a custom flow circuit was set up to emulate the physiological state of FPA in conjunction with the peristaltic flow at 100 mmHg. An IVUS catheter was introduced in a 66-year-old male FPA via a dry seal to ensure minimal leakage. The cross-sectional IVUS images were then recorded as the catheter was withdrawn at 0.5mm/s speed. After image acquisition, the artery was fixed with 10% neutral buffered formalin under luminal pressure for 4 hours and then for 24 hours without pressure in order to maintain the shape of the arterial sample. The artery was then scanned in with microCT, and transverse histological specimens were excised from several locations along the FPA length and stained with Movat's Pentachrome. The histology slides were then co-registered with both IVUS and microCT. Our analysis shows that we can successfully cross-reference images from IVUS, microCT, and conventional histology. In the future, this analysis will be performed on a large sample size of FPAs.
Scheduling
9:15-10:30 a.m., 10:45 a.m.-Noon, 1-2:15 p.m.
A methodology for co-registering the intravascular ultrasound (IVUS) images with the histology sections in the peripheral vasculature
MBSC Ballroom Poster # 1202 - G (Masters)
Peripheral vascular disease (PAD), observed primarily in the femoropopliteal artery (FPA), affects over 200 million people worldwide. This vascular disease reduces blood flow, claudication, and gangrene, which remains a substantial public health burden. Intravascular Ultrasound (IVUS) is an imaging technique that uses ultrasound waves to accurately visualize the vascular structure, providing information about the arterial plaque and its composition, which helps the clinician with diagnosis and choosing the appropriate treatment approach. Virtual Histology IVUS (VH-IVUS) provides a more detailed assessment of plaque composition through spectral analysis of high-frequency ultrasound signals compared to greyscale IVUS, which remains less accurate in differentiating the heterogeneous plaque compositions. The VH-IVUS has been studied extensively in coronary and carotid arteries. However, there has yet to be a large-scale validation study that cross-references IVUS observations with conventional histology images in FPA. In this preliminary study, we aimed to develop a setup to image FPAs ex vivo using IVUS, micro Computed Tomography (microCT), and conventional histology in a loading condition similar to in vivo. To achieve this goal, a custom flow circuit was set up to emulate the physiological state of FPA in conjunction with the peristaltic flow at 100 mmHg. An IVUS catheter was introduced in a 66-year-old male FPA via a dry seal to ensure minimal leakage. The cross-sectional IVUS images were then recorded as the catheter was withdrawn at 0.5mm/s speed. After image acquisition, the artery was fixed with 10% neutral buffered formalin under luminal pressure for 4 hours and then for 24 hours without pressure in order to maintain the shape of the arterial sample. The artery was then scanned in with microCT, and transverse histological specimens were excised from several locations along the FPA length and stained with Movat's Pentachrome. The histology slides were then co-registered with both IVUS and microCT. Our analysis shows that we can successfully cross-reference images from IVUS, microCT, and conventional histology. In the future, this analysis will be performed on a large sample size of FPAs.