Characterization of the Role of PA5189 of Pseudomonas aeruginosa in Resistance to an Antimicrobial Peptide
Presenter Type
UNO Undergraduate Student
Major/Field of Study
Biology
Other
Molecular and Biomedical Biology
Advisor Information
Donald Rowen
Location
MBSC Ballroom Poster # 305 - U
Presentation Type
Poster
Start Date
24-3-2023 10:30 AM
End Date
24-3-2023 11:45 AM
Abstract
The emergence of multidrug resistance in bacteria demands an alternative to standard antibiotics and small-molecule therapeutics. As an opportunistic pathogen, the gram-negative bacterial species Pseudomonas aeruginosa has been highlighted by the WHO, NIH, and CDC, among other organizations, as a pathogen with extreme economic and public health burdens for which there is an urgent demand for alternative therapies. Bacterial infections are often facilitated by pathogenic cellular processes, such as quorum sensing and biofilm formation, which are heavily regulated by transcription factors (TFs). As such, TFs may serve as possible drug targets for development of novel antimicrobial agents targeting cellular processes contributing to pathogenicity. Previous research has identified PA5189, a relatively unstudied TF in P. aeruginosa, as a possible regulator of virulence. The rationale behind this project was to investigate the role of PA5189 by characterizing its genetic and physiological impacts in PA5189 deletion and overexpression strains.
Scheduling
10:45 a.m.-Noon, 1-2:15 p.m.
Characterization of the Role of PA5189 of Pseudomonas aeruginosa in Resistance to an Antimicrobial Peptide
MBSC Ballroom Poster # 305 - U
The emergence of multidrug resistance in bacteria demands an alternative to standard antibiotics and small-molecule therapeutics. As an opportunistic pathogen, the gram-negative bacterial species Pseudomonas aeruginosa has been highlighted by the WHO, NIH, and CDC, among other organizations, as a pathogen with extreme economic and public health burdens for which there is an urgent demand for alternative therapies. Bacterial infections are often facilitated by pathogenic cellular processes, such as quorum sensing and biofilm formation, which are heavily regulated by transcription factors (TFs). As such, TFs may serve as possible drug targets for development of novel antimicrobial agents targeting cellular processes contributing to pathogenicity. Previous research has identified PA5189, a relatively unstudied TF in P. aeruginosa, as a possible regulator of virulence. The rationale behind this project was to investigate the role of PA5189 by characterizing its genetic and physiological impacts in PA5189 deletion and overexpression strains.